Around 1 a.m., Johnson City police were called to the eastbound lanes of the interstate near Exit 27, the Okolona Road exit.
When officers arrived at the scene, they found 28-year-old Brady K. Musick, of Vinton Avenue, Erwin, lying in a lane of traffic.
Musick, who reportedly worked as a security guard at Nuclear Fuel Services in Erwin, was pronounced dead at the scene.
According to reports, Musick likely wrecked his 2001 Harley-Davidson motorcycle when he either struck a deer or swerved to miss the animal.
A motorist traveling in the westbound lanes of I-26 spotted the bike lying on the shoulder of the road and pulled over to offer assistance.
That individual apparently was attempting to warn oncoming traffic that the motorcyclist was lying in the right-hand lane of traffic when a vehicle struck Musick and then fled the scene.
Moments later, a Ford Mustang and then a Chevrolet S10 pickup truck ran over Musick as he lay on the highway, police said.
Police believe Musick still was alive when he was struck by the first vehicle, and authorities are searching for the driver of that automobile, which was described as a dark-colored, midsize car.
Anyone with information as to the identity of the hit-and-run vehicle or driver is urged to contact the Johnson City Police Department at 434-6126.
An investigation into Tuesday's traffic fatality is being conducted by members of the JCPD's Traffic Homicide Team.